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Enhancing the efficiency of nitrogen removing bacterial population to a wide range of C:N ratio (1.5:1 to 14:1) for simultaneous C & N removal

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1522-y

摘要：

• Simultaneous C & N removal in Methammox occurs at wide C:N ratio.

关键词： Methanogens     Biological Nitrogen Removal (BNR)     Simultaneous     Methammox     C:N ratio    

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Experimental study of nitrite accumulation in pre-denitrification biological nitrogen removal process

WU Xuelei, CHEN Lunqiang, PENG Yongzhen, WANG Pu, WANG Yayi

《环境科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 236-240 doi: 10.1007/s11783-008-0008-x

摘要： The effect of dissolved oxygen (DO) concentration on nitrite accumulation was investigated in a pilot-scale pre-denitrification process at room temperature for 100 days. In the first 10 days, due to the instability of the system, the DO concentration fluctuated between 1.0 and 2.0 mg/L. In the next 14 days, the DO concentration was kept at 0.5 mg/L and nitrite accumulation occurred, with the average nitrite accumulation rate at 91%. From the 25th day, the DO concentration was increased to 2.0 mg/L to destroy the nitrite accumulation, but nitrite accumulation rate was still as high as 90%. From the 38th day the nitrite accumulation rate decreased to 15%–30% linearly. From the 50th day, DO concentration was decreased to 0.5 mg/L to resume nitrite accumulation. Until the 83rd day the nitrite accumulation rate began to increase to 80%. Dissolved oxygen was the main cause of nitrite accumulation, taking into account other factors such as pH, free ammonia concentration, temperature, and sludge retention time. Because of the different affinity for oxygen between nitrite oxidizing bacteria and ammonia oxidizing bacteria when DO concentration was kept at 0.5 mg/L, nitrite accumulation occurred.
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Circulating fluidized bed biological reactor for nutrients removal

CUI Yubo, LIU Hongbo, BAI Chunxue

《环境科学与工程前沿（英文）》 2008年 第2卷 第3期   页码 349-353 doi: 10.1007/s11783-008-0051-7

摘要： A new biological nitrogen removal process, which is named herein “The circulating fluidized bed bioreactor (CFBBR)”, was developed for simultaneous removal of nitrogen and organic matter. This process was composed of an anaerobic bed (Riser), aerobic bed (Downer) and connecting device. Influent and nitrified liquid from the aerobic bed enters the anaerobic bed from the bottom of the anaerobic bed, completing the removal of nitrogen and organic matter. The system performance under the conditions of different inflow loadings and nitrified liquid recirculation rates ranging from 200% to 600% was examined. From a technical and economic point of view, the optimum nitrified liquid recirculation rate was 400%. With a shortest total retention time of 2.5 h (0.8 h in the anaerobic bed and 1.5 h in the aerobic bed) and a nitrified liquid recirculation rate of 400% based on the influent flow rate, the average removal efficiencies of total nitrogen (TN) and soluble chemical oxygen demand (SCOD) were found to be 88% and 95%, respectively. The average effluent concentrations of TN and SCOD were 3.5 mg/L and 16 mg/L, respectively. The volatile suspended solid (VSS) concentration, nitrification rate and denitrification rate in the system were less than 1.0 g/L, 0.026–0.1 g NH-N/g VSSd, and 0.016–0.074 g NO-N/g VSSd, respectively.

关键词： soluble chemical     bioreactor     biological nitrogen     optimum nitrified     nitrogen removal    

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Feammox and anammox process facilitated by activated carbon as an electron shuttle for autotrophic biologicalnitrogen removal

《环境科学与工程前沿（英文）》 2022年 第16卷 第7期 doi: 10.1007/s11783-021-1498-z

摘要：

• The autotrophic nitrogen removal combining Feammox and Anammox was achieved.

关键词： Feammox     Anammox     Extracellular electron transfer     Electron shuttle     Activated carbon    

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Research on polyhydroxyalkanoates and glycogen transformations: Key aspects to biologic nitrogen andphosphorus removal in low dissolved oxygen systems

Hongjing LI, Yinguang CHEN

《环境科学与工程前沿（英文）》 2011年 第5卷 第2期   页码 283-290 doi: 10.1007/s11783-010-0243-9

摘要： In this paper, a study was conducted on the effect of polyhydroxyalkanoates (PHA) and glycogen transformations on biologic nitrogen and phosphorus removal in low dissolved oxygen (DO) systems. Two laboratory-scale sequencing batch reactors (SBR1 and SBR2) were operating with anaerobic/aerobic (low DO, 0.15–0.45 mg·L ) configurations, which cultured a propionic to acetic acid ratio (molar carbon ratio) of 1.0 and 2.0, respectively. Fewer poly-3-hydroxybutyrate (PHB), total PHA, and glycogen transformations were observed with the increase of propionic/acetic acid, along with more poly-3-hydroxyvalerate (PHV) and poly-3-hydroxy-2-methyvalerate (PH2MV) shifts. The total nitrogen (TN) removal efficiency was 68% and 82% in SBR1 and SBR2, respectively. In the two SBRs, the soluble ortho-phosphate (SOP) removal efficiency was 94% and 99%, and the average sludge polyphosphate (poly-P) content (g·g-MLVSS ) was 8.3% and 10.2%, respectively. Thus, the propionic to acetic acid ratio of the influent greatly influenced the PHA form and quantity, glycogen transformation, and poly-P contained in activated sludge and further determined TN and SOP removal efficiency. Moreover, significant correlations between the SOP removal rate and the (PHV+ PH2MV)/PHA ratio were observed ( >0.99). Accordingly, PHA and glycogen transformations should be taken into account as key components for optimizing anaerobic/aerobic (low DO) biologic nitrogen and phosphorus removal systems.

关键词： low dissolved oxygen (DO)     biological nitrogen and phosphorus removal     polyhydroxyalkanoates (PHA)     glycogen    

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Practical consideration for design and optimization of the step feed process

Shijian GE, Yongzhen PENG, Congcong LU, Shuying WANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 135-142 doi: 10.1007/s11783-012-0454-3

摘要： Based on the anoxic/oxic (A/O) step feed process, a modified University of Cape Town (UCT) step feed process was developed by adding an anaerobic zone and adjusting sludge return pipeline. Performance evaluation of these two types of processes was investigated by optimizing operational parameters, such as the anaerobic/anoxic/oxic volumes, internal recycle ratios, and sludge retention times, for removal of chemical oxygen demanding (COD), nitrogen, and phosphorus. Results showed high removal efficiencies of COD of (85.0±1.7)%, ammonium of (99.7±0.2)%, total nitrogen (TN) of (85.5±1.7)%, phosphorus of (95.1±3.3)%, as well as excellent sludge settleability with average sludge volume index of (83.7±9.5) L·mg in the modified UCT process. Moreover, (61.5±6.0)% of influent COD was efficiently involved in denitrification or phosphorus release process. As much as 35.3% of TN was eliminated through simultaneous nitrification and denitrification process in aerobic zones. In addition, the presence of denitrifying phosphorus accumulating organisms (DNPAOs), accounting for approximately 39.2% of PAOs, was also greatly beneficial to the nitrogen and phosphorus removal. Consequently, the modified UCT step feed process was more attractive for the wastewater treatment plant, because it had extremely competitive advantages such as higher nutrient removal efficiencies, lower energy and dosages consumption, excellent settling sludge and operational assurance.

关键词： step feed     anoxic/oxic (A/O)     University of Cape Town (UCT)     simultaneous nitrification and denitrification (SND)     denitrifying phosphorus removal     biological nutrient removal (BNR)    

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On-line controlling system for nitrogen and phosphorus removal of municipal wastewater in a sequencing

LI Jun, NI Yongjiong, WEI Su, CHENG Guobiao, OU Changjin, PENG Yongzhen, GU Guowei, LU Jingen

《环境科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 99-102 doi: 10.1007/s11783-008-0017-9

摘要： The objectives of this study were to establish an on-line controlling system for nitrogen and phosphorus removal synchronously of municipal wastewater in a sequencing batch reactor (SBR). The SBR for municipal wastewater treatment was operated in sequences: filling, anaerobic, oxic, anoxic, oxic, settling and discharge. The reactor was equipped with on-line monitoring sensors for dissolved oxygen (DO), oxidation-reduction potential (ORP) and pH. The variation of DO, ORP and pH is relevant to each phase of biological process for nitrogen and phosphorus removal in this SBR. The characteristic points of DO, ORP and pH can be used to judge and control the stages of process that include: phosphate release by the turning points of ORP and pH; nitrification by the ammonia valley of pH and ammonia elbows of DO and ORP; denitrification by the nitrate knee of ORP and nitrate apex of pH; phosphate uptake by the turning point of pH; and residual organic carbon oxidation by the carbon elbows of DO and ORP. The controlling system can operate automatically for nitrogen and phosphorus efficiently removal.

关键词： DO     relevant     biological process     nitrogen     pH    

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Field experiment on biological contact oxidation process to treat polluted river water in the Dianchi

Lu LI, Shuguang XIE, Hui ZHANG, Donghui WEN

《环境科学与工程前沿（英文）》 2009年 第3卷 第1期   页码 38-47 doi: 10.1007/s11783-009-0007-6

摘要： In this study two types of biological contact oxidation processes (BCOP), a step-feed (SBCOP) unit and an inter-recycle (IBCOP) unit, were designed to investigate the treatment of heavily polluted river water. The Daqing River, which is the largest pollutant contributor to the Dianchi Lake, one of the most eutrophic freshwater lakes in China, was taken for the case study. It was found that the SBCOP had higher adaptability and better performance in the reduction of COD, TN, and TP, which made it applicable for the treatment of polluted river water entering the Dianchi Lake. Nitrification rate was observed to be greatly affected by the influent temperature. During each season, the nitrification in the SBCOP was higher than that in the IBCOP. TN removal efficiency in the SBCOP was higher than that in the IBCOP during the winter and spring but poorer during the summer, possibly due to the inhibition of denitrification by higher dissolved oxygen level in the summer. Moreover, symbiotic algae-bacteria growth may be conducive to the removal of pollutants.

关键词： step-feed biological contact oxidation process (SBCOP)     inter-recycle biological contact oxidation process (IBCOP)     river water     removal efficiency     nitrogen transformation     the Dianchi Lake watershed    

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Effects of mulching for water conservation on soil carbon, nitrogen and biological properties

Xiaomin PI, Tongxun ZHANG, Benhua SUN, Quanhong CUI, Yun GUO, Mingxia GAO, Hao FENG, David W. HOPKINS

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 146-154 doi: 10.15302/J-FASE-2017136

摘要： The effect of mulching with straw, white plastic film and black plastic film for improving soil water storage and on the soil properties was examined in a wheat-maize rotation system on Loess soil in northwestern China. All the mulches improved the water storage to some extent and led to warmer soils. However, the organic C and total N contents of the soils declined significantly under the mulches, particularly the plastic film mulches, and this could have long-term detrimental effects on soil quality and the sustainability of the some mulching practices.

关键词： biomass     carbon     mulch     nitrogen     plastic films     soil     straw     water storage    

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Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1426-2

摘要：

• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.

关键词： Ammonia oxidizing archaea (AOA)     Complete ammonia oxidizing (comammox) bacteria     Dissimilatory nitrate reduction to ammonium (DNRA) bacteria     Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms     Engineering application    

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ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING

《农业科学与工程前沿（英文）》 2022年 第9卷 第2期   页码 177-186 doi: 10.15302/J-FASE-2021421

摘要：

A range of plant species produce root exudates that inhibit ammonia-oxidizing microorganisms. This biological nitrification inhibition (BNI) capacity can decrease N loss and increase N uptake from the rhizosphere. This study sought evidence for the existence and magnitude of BNI capacity in canola ( Brassica napus). Seedlings of three canola cultivars, Brachiaria humidicola(BNI positive) and wheat ( Triticum aestivum) were grown in a hydroponic system. Root exudates were collected and their inhibition of the ammonia oxidizing bacterium, Nitrosospira multiformis, was tested. Subsequent pot experiments were used to test the inhibition of native nitrifying communities in soil. Root exudates from canola significantly reduced nitrification rates of both N. multiformis cultures and native soil microbial communities. The level of nitrification inhibition across the three cultivars was similar to the well-studied high-BNI species B. humidicola. BNI capacity of canola may have implications for the N dynamics in farming systems and the N uptake efficiency of crops in rotational farming systems. By reducing nitrification rates canola crops may decrease N losses, increase plant N uptake and encourage microbial N immobilization and subsequently increase the pool of organic N that is available for mineralization during the following cereal crops.

关键词： ammonia oxidizing microorganisms / biological nitrification inhibition / farming rotations / nitrogen cycling / nitrogen use efficiency    

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Enhanced nitrogen removal reliability and efficiency in integrated constructed wetland microcosms using

Yue WEN, Chao XU, Gang LIU, Yi CHEN, Qi ZHOU

《环境科学与工程前沿（英文）》 2012年 第6卷 第1期   页码 140-147 doi: 10.1007/s11783-011-0286-6

摘要： The purpose of this study is to reduce the seasonal fluctuation and enhance the efficiency of nitrogen removal in vertical flow-horizontal subsurface flow (VF-HSF) constructed wetlands. Two sets of VF-HSF constructed wetlands were built, VF1-HSF1 and VF2-HSF2, and a zeolite section was placed in VF2. The results showed that VF2-HSF2 compared to VF1-HSF1 was not only a more reliable nitrogen removal method, but also enhanced the nitrogen removal efficiency by 50%. The average apparent rate of nitrogen removal in VF2-HSF2 reached to 2.52 gN·m ·d , which doubled the rate in VF1-HSF1. Plant uptake and organic nitrogen sediment accounted for 12% and 6% of the total nitrogen removal in VF1-HSF1, respectively, and 10% and 4% in VF2-HSF2, respectively. Biologic nitrogen removal was the dominant mechanism, which accounted for 79% and 87% of the total nitrogen removal in VF1-HSF1 and VF2-HSF2, respectively. Ammonia adsorbed by zeolite during the cold seasons was desorbed, and then nitrified in warm seasons, which resulted in a bioregeneration efficiency of 91%. Zeolite in VF was capable of transferring ammonia from cold seasons to warm seasons as well as enhancing nitrification, which was accompanied by high potential denitrification in HSF that reinforced the efficiency and relieved seasonal fluctuation of nitrogen removal in VF-HSF.

关键词： constructed wetland     zeolite     bioregeneration     nitrogen removal    

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Advanced nitrogen and phosphorus removal in A

Jianhua WANG, Yongzhen PENG, Yongzhi CHEN

《环境科学与工程前沿（英文）》 2011年 第5卷 第3期   页码 474-480 doi: 10.1007/s11783-011-0360-0

摘要： A laboratory-scale anaerobic-anoxic-aerobic process (A O) with a small aerobic zone and a bigger anoxic zone and biologic aerated filter (A O-BAF) system was operated to treat low carbon-to-nitrogen ratio domestic wastewater. The A O process was employed mainly for organic matter and phosphorus removal, and for denitrification. The BAF was only used for nitrification which coupled with a settling tank Compared with a conventional A O process, the suspended activated sludge in this A O-BAF process contained small quantities of nitrifier, but nitrification overwhelmingly conducted in BAF. So the system successfully avoided the contradiction in sludge retention time (SRT) between nitrifying bacteria and phosphorus accumulating organisms (PAOs). Denitrifying phosphorus accumulating organisms (DPAOs) played an important role in removing up to 91% of phosphorus along with nitrogen, which indicated that the suspended activated sludge process presented a good denitrifying phosphorus removal performance. The average removal efficiency of chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), and were 85.56%, 92.07%, 81.24% and 98.7% respectively. The effluent quality consistently satisfied the national first level A effluent discharge standard of China. The average sludge volume index (SVI) was 85.4 mL·g additionally, the volume ratio of anaerobic, anoxic and aerobic zone in A O process was also investigated, and the results demonstrated that the optimum value was 1∶6∶2.

关键词： Anoxic zone and biologic aerated filter (A2O-BAF) system     domestic wastewater with low carbon-to-nitrogen ratio     advanced nitrogen and phosphorus removal     denitrifying phosphorus removal    

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Methanation and chemolitrophic nitrogen removal by an anaerobic membrane bioreactor coupled partial nitrification

《环境科学与工程前沿（英文）》 2023年 第17卷 第6期 doi: 10.1007/s11783-023-1668-2

摘要：

● Efficient carbon methanation and nitrogen removal was achieved in AnMBR-PN/A system.

关键词： Anaerobic membrane bioreactor     Partial nitrification/Anammox     Carbon separation     Chemolitrophic nitrogen removal    

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Impact of photosynthesis and transpiration on nitrogen removal in constructed wetlands

LUO Weiguo, WANG Shihe, HUANG Juan, YAN Lu, HUANG Jun

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 316-319 doi: 10.1007/s11783-007-0053-x

摘要： To determine the impact of photosynthesis and transpiration on nitrogen removal in wetlands, an artificial wetland planted with reeds was constructed to treat highly concentrated domestic wastewater. Under different meteorological and hydraulic conditions, the daily changes of photosynthesis and transpiration of reeds, as well as nitrogen removal efficiency were measured. It was found that net photosynthesis rate per unit leaf area was maintained on a high level (average 19.0 yDol CO/(m · s) ) from 10:00 to 14:00 in July 2004 and reached a peak of 21.1 μmol CO/(m · s) when Photon Flux Density was high during the day. Meanwhile, TN and NH-N removal efficiency rose to 79.6% and 89.6%, respectively–the maximum values observed in the test. Correlation coefficient analysis demonstrated a positive correlation among photon flux density, net photosynthetic rate, transpiration rate, and TN and NH-N removal efficiency. In contrast, there was a negative correlation between stomatal conductance and TN and NH-N removal efficiency. Results suggest that the photosynthesis and transpiration of wetland plants have a great impact on nitrogen removal efficiency of wetlands, which can be enhanced by an increase in the photosynthesis and transpiration rate. In addition, the efficiency of water usage by reeds and nitrogen removal efficiency could be affected by the water level in wetlands; a higher level boosts nitrogen removal efficiency.

关键词： transpiration     Correlation coefficient     nitrogen removal     artificial wetland     impact    

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标题 作者 时间 类型 操作

Enhancing the efficiency of nitrogen removing bacterial population to a wide range of C:N ratio (1.5:1 to 14:1) for simultaneous C & N removal

期刊论文

Experimental study of nitrite accumulation in pre-denitrification biological nitrogen removal process

WU Xuelei, CHEN Lunqiang, PENG Yongzhen, WANG Pu, WANG Yayi

期刊论文

Circulating fluidized bed biological reactor for nutrients removal

CUI Yubo, LIU Hongbo, BAI Chunxue

期刊论文

Feammox and anammox process facilitated by activated carbon as an electron shuttle for autotrophic biologicalnitrogen removal

期刊论文

Research on polyhydroxyalkanoates and glycogen transformations: Key aspects to biologic nitrogen andphosphorus removal in low dissolved oxygen systems

Hongjing LI, Yinguang CHEN

期刊论文

Practical consideration for design and optimization of the step feed process

Shijian GE, Yongzhen PENG, Congcong LU, Shuying WANG

期刊论文

On-line controlling system for nitrogen and phosphorus removal of municipal wastewater in a sequencing

LI Jun, NI Yongjiong, WEI Su, CHENG Guobiao, OU Changjin, PENG Yongzhen, GU Guowei, LU Jingen

期刊论文

Field experiment on biological contact oxidation process to treat polluted river water in the Dianchi

Lu LI, Shuguang XIE, Hui ZHANG, Donghui WEN

期刊论文

Effects of mulching for water conservation on soil carbon, nitrogen and biological properties

Xiaomin PI, Tongxun ZHANG, Benhua SUN, Quanhong CUI, Yun GUO, Mingxia GAO, Hao FENG, David W. HOPKINS

期刊论文

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

期刊论文

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING

期刊论文

Enhanced nitrogen removal reliability and efficiency in integrated constructed wetland microcosms using

Yue WEN, Chao XU, Gang LIU, Yi CHEN, Qi ZHOU

期刊论文

Advanced nitrogen and phosphorus removal in A

Jianhua WANG, Yongzhen PENG, Yongzhi CHEN

期刊论文

Methanation and chemolitrophic nitrogen removal by an anaerobic membrane bioreactor coupled partial nitrification

期刊论文

Impact of photosynthesis and transpiration on nitrogen removal in constructed wetlands

LUO Weiguo, WANG Shihe, HUANG Juan, YAN Lu, HUANG Jun

期刊论文